> ## Documentation Index
> Fetch the complete documentation index at: https://wiki.latch.bio/llms.txt
> Use this file to discover all available pages before exploring further.
# Overview - MAGeCK
> Model-based Analysis of Genome-wide CRISPR-Cas9 Knockout
[MAGeCK](https://genomebiology.biomedcentral.com/articles/10.1186/s13059-014-0554-4) is a computational tool to identify important genes from the recent genome-scale CRISPR-Cas9 knockout screens (or GeCKO) technology. MAGeCK can be used for prioritizing single-guide RNAs, genes and pathways in genome-scale CRISPR/Cas9 knockout screens. MAGeCK identifies both positively and negatively selected genes simultaneously and reports robust results across different experimental conditions. MAGeCK is developed and maintained by Wei Li and Han Xu from [Prof. Xiaole Shirley Liu's lab](http://liulab.dfci.harvard.edu/) at the Department of Biostatistics and Computational Biology, Dana-Farber Cancer Institute and Harvard School of Public Health. MAGeCK has been used to identify functional lncRNAs from screens with close to [100% validation rate](https://sourceforge.net/p/mageck/wiki/Home/).
## How to run MAGeCK on Latch
On Latch we have split up the MAGeCK workflow into its subcommands to be run. These are:
You can get an overview on how these subcommands work and feed into one another through this graph:
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